AMPK alpha1/2 Polyclonal Antibody

Specifications

Catalog number

E-AB-30491-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.

Abbreviation

AMPKα1/2

UniProt

Q13131, P54646

Host

Rabbit

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

Synthesized peptide derived from human AMPKα1/2 around the non-phosphorylation site of Thr183/172.

Target

PRKAA1; AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC-p

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:100-1:300

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

62kDa

Observed Molecular Weight

63kDa

Isotype

IgG