JAK2 Polyclonal Antibody

Specifications

Catalog number

E-AB-31844-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Non-receptor tyrosine kinase involved in various processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and histone modifications. In the cytoplasm, plays a pivotal role in signal transduction via its association with cytokine receptors, which constitutes an initiating step in signaling for many members of the cytokine receptor superfamily including the receptors for growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), granulocyte-macrophage colony-stimulating factor (CSF2), thrombopoietin (THPO) and multiple interleukins. Following stimulation with erythropoietin (EPO) during erythropoiesis, it is autophosphorylated and activated, leading to its association with erythropoietin receptor (EPOR) and tyrosine phosphorylation of residues in the EPOR cytoplasmic domain. Also involved in promoting the localization of EPOR to the plasma membrane. Also acts downstream of some G-protein coupled receptors. Plays a role in the control of body weight (By similarity) . Mediates angiotensin-2-induced ARHGEF1 phosphorylation. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Abbreviation

JAK2

UniProt

O60674

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human JAK2 around the non-phosphorylation site of Tyr1007.

Target

JAK 2; JAK-2; JAK2; JAK2; Janus Activating Kinase 2; Janus kinase 2 (a protein tyrosine kinase) ; Janus kinase 2; JTK 10; JTK10; kinase Jak2; OTTHUMP00000043260; THCYT3; Tyrosine protein kinase JAK2; Tyrosine-protein kinase JAK2

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC-p; IF

Field of Research

Cancer; Cell biology; Epigenetics and Nuclear Signaling; Signal transduction

Purification

Affinity purification

Concentration

1mg/mL

Dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:50-1:200

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

131kDa

Observed Molecular Weight

130kDa

Citation 01

https://journals.sagepub.com/doi/abs/10.1177/1758835919846806

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acs.jnatprod.9b00145

Isotype

IgG