NFκB-p65 Polyclonal Antibody

Specifications

Catalog number

E-AB-32233-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

Abbreviation

NFκB-p65

UniProt

Q04206

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human NFκB-p65 around the non-phosphorylation site of Ser281.

Target

Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NF kappa B p65delta3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; OTTHUMP00000233473; OTTHUMP00000233474; OTTHUMP00000233475; OTTHUMP00000233476; OTTHUMP00000233900; p65; p65 NF kappaB; p65 NFkB; relA; TF65; Transcription factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65) ) ; V rel avian reticuloendotheliosis viral oncogene homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian) ; V rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B cells 3; p65

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC-p

Field of Research

Cancer; Cell Biology; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Neuroscience; Signal Transduction

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:100-1:300

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

60kDa

Observed Molecular Weight

60kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S0144861722009523

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332223000823

Citation 03

https://journals.lww.com/cmj/Fulltext/9900/Human_papillomavirus,_tobacco,_and_poor_oral.47.aspx

Citation 04

https://www.sciencedirect.com/science/article/pii/S0006295221003336

Citation 05

https://europepmc.org/article/pmc/pmc8333701?javascript_support=no

Citation 06

https://www.sciencedirect.com/science/article/pii/S240584401936181X

Isotype

IgG