SMC1A Polyclonal Antibody

Specifications

Catalog number

E-AB-52219-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Abbreviation

SMC1A

UniProt

Q14683

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Fusion protein of human SMC1A

Target

Chromosome segregation protein SmcB; DXS423E; KIAA0178; MGC138332; Sb1.8; Segregation of mitotic chromosomes 1; SMC protein 1A; SMC-1-alpha; SMC-1A; SMC1 (structural maintenance of chromosomes 1 yeast) like 1; SMC1; SMC1 structural maintenance of chromosomes 1 like 1; SMC1A; SMC1A; SMC1alpha; SMC1L1; SMCB; Structural maintenance of chromosomes 1A; Structural maintenance of chromosomes protein 1A

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Cancer; Cell Biology

Purification

Antigen affinity purification

Concentration

0.5 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:25-1:100

Buffer

PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

143 kDa

Observed Molecular Weight

Refer to figures

Isotype

IgG