Caspase-8 Polyclonal Antibody

Specifications

Catalog number

E-AB-62025-01

Size / count

60 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Abbreviation

Caspase-8

UniProt

Q14790

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant fusion protein of human Caspase-8 (NP_001073593.1) .

Target

ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; Caspase 8; CASP8; Caspase-8; caspase-8

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Cancer; Cell Biology; Metabolism

Purification

Affinity purification

Concentration

1mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

25-32kDa/45- 61kDa

Observed Molecular Weight

17kDa/44kDa/60kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332221003772

Citation 02

https://www.mdpi.com/article/10.3390/plants12030529

Citation 03

https://www.mdpi.com/2072-6651/11/9/506

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/13880209.2020.1765812

Isotype

IgG