DLAT Polyclonal Antibody

Specifications

Catalog number

E-AB-66302-01

Size / count

60 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC) . PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC) . In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

Abbreviation

DLAT

UniProt

P10515

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant fusion protein of human DLAT (NP_001922.2) .

Target

DLAT; DLTA; PDC-E2; PDCE2

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Cancer; Metabolism; Signal transduction

Purification

Affinity purification

Concentration

1mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:100; IF 1:50-1:200

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

68kDa

Observed Molecular Weight

69kDa

Isotype

IgG