GPX4 Polyclonal Antibody

Specifications

Catalog number

E-AB-67390-01

Size / count

60 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS) . This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Abbreviation

GPX4

UniProt

P36969

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant fusion protein of human GPX4

Target

GPX4; GPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IF

Field of Research

Cancer; Metabolism; Signal Transduction

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IF 1:50-1:200

Buffer

PBS with 0.05% proclin300,50% glycerol, pH7.3.

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

19kDa/22kDa

Observed Molecular Weight

22KDa

Isotype

IgG