RBFOX3 Polyclonal Antibody

Specifications

Catalog number

E-AB-70267-01

Size / count

60 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.

Abbreviation

RBFOX3

UniProt

Q8BIF2

Host

Rabbit

Reactivity

Mouse; Rat

Immunogen

KLH conjugated Synthetic peptide corresponding to NeuN

Target

FLJ56884; FLJ58356; Fox-1 homolog C; fox1 homolog C; Fox3; FOX3NeuN; hexaribonucleotide binding protein 3; HRNBP3; NEUN; neuronal nuclei; Rbfox3; RFOX3; RNA binding protein fox-1 homolog 3; RNA binding protein; fox 1 homolog (C. elegans) 3

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Cancer; Epigenetics and Nuclear Signaling; Neuroscience; Tags and Cell Markers

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:300-1:800

Buffer

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

46-48kDa

Observed Molecular Weight

46-48kDa

Citation 01

https://academic.oup.com/jnen/article-abstract/81/8/596/6633607

Isotype

IgG