Recombinant Alkaline Phosphatase Monoclonal Antibody

Specifications

Catalog number

E-AB-81527-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific) . The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.

Abbreviation

Alkaline Phosphatase

UniProt

P05186

Host

Rabbit

Reactivity

Human; Rat

Immunogen

A synthetic peptide of human Alkaline Phosphatase, Tissue Non-Specific

Target

AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase; tissue-nonspecific isozyme; Alkaline phosphomonoesterase; Alpl; AP TNAP; AP-TNAP; APTNAP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP

Clonality

Monoclonal

Clone

R08-6H0

Conjugation

Unconjugated

Applications

WB; IHC-P; IF

Field of Research

Cancer; Cell Biology; Tags and Cell Markers

Purification

Affinity Purified

Concentration

300 μg/mL

Dilution

WB 1:1000-1:2000; IHC 1:50-1:100; IF 1:50-1:100

Buffer

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

57kDa

Observed Molecular Weight

80kDa

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/jor.23245

Isotype

IgG