Recombinant cAMP Protein Kinase Catalytic Subunit Monoclonal Antibody

Specifications

Catalog number

E-AB-81539-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Abbreviation

Recombinant cAMP Protein Kinase Catalytic Subunit

UniProt

P17612

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

A synthetic peptide of human cAMP Protein Kinase Catalytic subunit

Target

CAMP dependent protein kinase alpha catalytic subunit; cAMP dependent protein kinase beta catalytic subunit; cAMP dependent protein kinase catalytic beta subunit isoform 4ab; cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit alpha; isoform 1; cAMP dependent protein kinase catalytic subunit beta; cAMP-dependent protein kinase catalytic subunit alpha; KAPCA; PKA C alpha; PKA C beta; PKA C-alpha; PKACA; PKACB; PPNAD4; PRKACA; PRKACAA; PRKACB; Protein kinase A catalytic subunit alpha; Protein kinase A catalytic subunit; Protein kinase A catalytic subunit beta; Protein kinase cAMP dependent catalytic alpha; Protein kinase cAMP dependent catalytic beta

Clonality

Monoclonal

Clone

R07-8H3

Conjugation

Unconjugated

Applications

WB; IHC-P; IF; IP

Field of Research

Cancer; Metabolism; Signal Transduction

Purification

Affinity Purified

Concentration

300 μg/mL

Dilution

WB 1:1000-1:2000; IHC 1:50-1:100; IF 1:50-1:100

Buffer

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

41kDa

Observed Molecular Weight

41kDa

Isotype

IgG