AIF Polyclonal Antibody

Specifications

Catalog number

E-AB-90029-01

Size / count

120 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.

Abbreviation

AIF

UniProt

O95831

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant fusion protein of human AIF

Target

AIF; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; AIFM1

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Metabolism

Purification

Affinity purification

Concentration

1mg/mL

Dilution

WB 1:500-1:1000; IHC 1:50-1:200; IF 1:20-1:50

Buffer

PBS with 0.02% sodium azide,50% glycerol, pH7.3.

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

26kDa/28kDa/35kDa/66kDa

Observed Molecular Weight

60kDa

Isotype

IgG