Anti-MERS-CoV Nucleocapsid Monoclonal Antibody

Specifications

Catalog number

E-AB-V1281-01

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Host

Mouse

Reactivity

MERS-CoV

Immunogen

Recombinant MERS-CoV Nucleoprotein / NP protein (His Tag)

Target

Coronavirus NP; coronavirus Nucleocapsid; coronavirus Nucleoprotein; cov np; ncov NP; novel coronavirus Nucleoprotein; NP; Nucleocapsid; Nucleoprotein

Clonality

Monoclonal

Clone

10

Conjugation

Unconjugated

Applications

WB; ELISA

Purification

Protein A Affinity

Concentration

1mg/mL

Dilution

WB 1:1000-1:5000

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Isotype

IgG1