Anti-MERS-CoV (NCoV/Novel coronavirus) Spike Monoclonal Antibody

Specifications

Catalog number

E-AB-V1302-01

Size / count

200 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Host

Rabbit

Reactivity

MERS-CoV

Immunogen

Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)

Target

Coronavirus s1; coronavirus s2; coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; RBD; S; s1; Spike RBD

Clonality

Monoclonal

Clone

723

Conjugation

Unconjugated

Applications

Microneutralizaiton; ELISA

Purification

Protein A Affinity

Concentration

1mg/mL

Dilution

ELISA 1:1000-10000

Buffer

0.2 μm filtered solution in PBS

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Isotype

IgG