Recombinant Human MCEE Protein (His Tag)

Specifications

Catalog number

PDMH100013-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Methylmalonyl-CoA epimerase, mitochondrial (MCEE) is an enzyme which belongs to the glyoxalase I family. It converts (S) -methylmalonyl-CoA to the (R) form, catalyses the following chemical reaction: (R) -methylmalonyl-CoA (S) -methylmalonyl-CoA. It plays an important role in the catabolism of fatty acids with odd-length carbon chains. This protein deficiency is an autosomal recessive inborn error of AA metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria can appear in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma.

Abbreviation

MCEE

Synonyms

DL-methylmalonyl-CoA racemase; Methylmalonyl-CoA epimerase; mitochondrial

UniProt

Q96PE7

Accession Number

Q96PE7

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln37-Ala176

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

16.0 kDa

Observed Molecular Weight

18-20 kDa

Species

Human