Recombinant Human Beta-glucuronidase/GUSB protein (His tag)

Specifications

Catalog number

PDMH100079-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human beta-Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan. The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits. Mutations in the GUSB gene are linked to mucopolysaccharidosis type VII. Accumulation of partially degraded glycosaminoglycans, with glucuronic acid residues at the non-reducing termini, are usually found in the lysosomes of patients with the disease. It has also been reported that this enzyme may contribute to the depletion of chondroitin from cartilage and thereby facilitate the damage of joints in rheumatoid arthritis.

Abbreviation

Beta-glucuronidase; GUSB

Synonyms

Beta-G1; Beta-glucuronidase; EC 3.2.1.31; GUSB

UniProt

P08236

Accession Number

P08236

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Thr651

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

71.5 kDa

Observed Molecular Weight

80 kDa

Species

Human