Recombinant Human EGFR/ErbB1 protein (His tag)

Specifications

Catalog number

PDMH100085-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The EGFR subfamily of receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43%-44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.

Abbreviation

EGFR; ErbB1

Synonyms

EGFR; ERBB; ERBB1; Epidermal growth factor receptor; HER1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

UniProt

P00533

Accession Number

P00533

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Gly640

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

70.3 kDa

Observed Molecular Weight

90 kDa

Species

Human