Recombinant Human WISP1 protein (His tag)

Specifications

Catalog number

PDMH100090-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X (L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.

Abbreviation

WISP1

Synonyms

CCN family member 4; CCN4; WISP-1; WISP1; WNT1-inducible-signaling pathway protein 1; Wnt-1-induced secreted protein

UniProt

O95388

Accession Number

O95388

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Asn367

Field of Research

Epigenetics and Nuclear Signaling

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.3 kDa

Observed Molecular Weight

60 kDa

Species

Human