Recombinant Human IGFBP-7 protein (His tag)

Specifications

Catalog number

PDMH100099-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Insulin-like growth factor-binding protein 7 (IGFBP-7) is a secreted glycosylated protein that contains three protein domain modules. IGFBP7 contains an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal immunoglobulin-like C2-type domain. Human and mouse IGFBP7 are highly homologous and share 94% aa sequence identity. It is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. It binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP7 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. It supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2. It modulates tumor cell growth and has also been implicated in angiogenesis.

Abbreviation

IGFBP-7

Synonyms

IGF-binding protein 7; IGFBP-rP1; IGFBP7; Insulin-like growth factor-binding protein 7; MAC25 protein; TAF; Tumor-derived adhesion factor

UniProt

Q16270

Accession Number

Q16270

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Leu282

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.9 kDa

Observed Molecular Weight

32 kDa

Species

Human