Recombinant Human Deoxyribonuclease 1/DNASE1 protein (His tag)

Specifications

Catalog number

PDMH100102-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.

Abbreviation

Deoxyribonuclease 1; DNASE1

Synonyms

DNASE1; DNL1; DNase I; DRNI; Deoxyribonuclease I; Deoxyribonuclease-1; Dornase alfa; EC 3.1.21.1

UniProt

P24855

Accession Number

P24855

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Lys282

Field of Research

Cell biology; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

30.9 kDa

Observed Molecular Weight

40 kDa

Species

Human