Recombinant Human CD32b/FCGR2B protein (His tag)

Specifications

Catalog number

PDMH100113-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

FcγRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcγRIIA, FcγRIIB, and FcγRIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcγRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcγRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcγRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcγRIIB on dendritic cells inhibits maturation and blocks cell activation. FcγRIIB may also be a target for monoclonal antibody therapy for malignancies. FcγRIIB plays an important negative-regulating role through modulating the signals from activation receptors.

Abbreviation

CD32b; FCGR2B

Synonyms

CD32; CDw32; FCG2; FCGR2B; Fc-Gamma RII-b; Fc-Gamma-RIIb; FcRII-b; IGFR2; IgG Fc Receptor II-b; Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b

UniProt

P31994

Accession Number

P31994

Expression System

HEK293 Cells

Tag

C-His

Sequence

Thr43-Pro217

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.1 kDa

Observed Molecular Weight

30 kDa

Species

Human