Recombinant Human Apolipoprotein E/ApoE protein (His tag)

Specifications

Catalog number

PDMH100121-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ApoE, a glycoprotein, is a structural component of very low density lipoprotein (vLDL) synthesized by the liver and intestinally synthesized chylomicrons . ApoE is also a constituent of a subclass of high density of lipoproteins (HDL) involved in cholesterol transport .ApoE mediates high affinity binding of chylomicrons and vLDL particles to the LDL receptor, allowing for specific uptake of these particles by the liver, preventing the accumulation of cholesterol rich particles in the plasma .Apolipoprotein E combines with fats (lipids) in the body to form molecules called lipoproteins and Apolipoprotein E is a major component of a specific type of lipoprotein called very low-density lipoproteins (VLDLs) .

Abbreviation

Apolipoprotein E; ApoE

Synonyms

APOE; Apo-E; Apolipoprotein E

UniProt

P02649

Accession Number

P02649

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-His317

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; metabolism; Stem cells

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.8 kDa

Observed Molecular Weight

38 kDa

Species

Human