Recombinant Human LILRB5/CD85c protein (His tag)

Specifications

Catalog number

PDMH100136-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 5 (LILRB5/CD85c/LIR-8) belongs to a family of transmembrane glycoproteins that negatively regulate immune cell activation. Mature human LIR-8 consists of a 435 amino acid (aa) extracellular domain with four Iglike domains, a 21 aa transmembrane segment, and a 111 aa cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM) . Alternative splicing of human LIR-8 generates an isoform that lacks the second Ig-like domain. LIR-8 is expressed on NK cells and in the tryptic granules of mast cells. Following cell activation and degranulation, it is present on the mast cell surface. Activated mast cells may also release soluble forms of LIR-8.

Abbreviation

LILRB5; CD85c

Synonyms

CD85 antigen-like family member C; CD85c; LILRB5; LIR-8; LIR8; Leukocyte immunoglobulin-like receptor 8; Leukocyte immunoglobulin-like receptor subfamily B member 5

UniProt

O75023

Accession Number

O75023

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Gly458

Field of Research

Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.3 kDa

Observed Molecular Weight

70 kDa

Species

Human