Recombinant Human CD80 protein (His tag)

Specifications

Catalog number

PDMH100154-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cluster of Differentiation 80, also called B7-1, is a member of cell surface immunoglobulin superfamily which plays key, yet distinct roles in the activation of T cells. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. Studies have shown that CTLA-4 binds mostly to CD80. The structure presents two extracellular domains: a membrane distal variable-like domain (IgV) and a membrane proximal Ig constant-like domain (IgC) along with an intracellular domain. Both IgV and IgC consist of anti-parallel beta sandwiches joined by a short linker region. CD80 is mostly expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells.

Abbreviation

CD80

Synonyms

Activation B7-1 antigen; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD28LGB7-1 antigen; CD80; LAB7; T-lymphocyte activation antigen CD80

UniProt

P33681

Accession Number

P33681

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Asn242

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.5 kDa

Observed Molecular Weight

40 kDa

Species

Human