Recombinant Mouse CD8a/Lyt2 protein (truncated) (His tag)

Specifications

Catalog number

PDMM100021-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B-and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Abbreviation

CD8a; Lyt2

Synonyms

BB154331; CD antigen CD8a; Ly-2; Ly-35; Ly-B; Lyt-2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2

UniProt

P01731

Accession Number

P01731

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Gly182

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.9 kDa

Observed Molecular Weight

35 kDa

Species

Mouse