Recombinant Rabbit IL10 protein (His tag)

Specifications

Catalog number

PDMO100002-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.

Abbreviation

IL-10

Synonyms

Interleukin; RP11-262N; CSIF; GVHDS; IL-10; IL10A; TGIF; IL10; Cytokine synthesis inhibitory factor; Interleukin-10; MGC126450; MGC126451; RP11-262N9.1

UniProt

Q9TSJ4

Accession Number

Q9TSJ4

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Ser178

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.6 kDa

Observed Molecular Weight

17 kDa

Species

Rabbit