Recombinant Rat AST protein (His tag)

Specifications

Catalog number

PDMR100007-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST) . Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Abbreviation

AST

Synonyms

Aspartate aminotransferase; Cysteine aminotransferase; Cysteine transaminase; Glutamate oxaloacetate transaminase 1; Got1; Transaminase A; cAspAT; cCAT; cytoplasmic

UniProt

P13221

Accession Number

P13221

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Gln413

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.3 kDa

Observed Molecular Weight

45 kDa

Species

Rat