Recombinant Rat CCl2 protein (His tag)

Specifications

Catalog number

PDMR100017-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein (MCP) -1 and small inducible cytokine A2 (SCYA2) ), is a small cytokine that belongs to the CC chemokine family responsible for monocyte attraction. Its cognate receptor, CCR2, play a critical role in regulating nociceptive processes during neuropathic pain. Both CCL2 and CCR2 are implicated in induction of autoimmunity. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Recently research also showed that CCL2 might be useful as a biomarker of fibrosis as well as a target for therapeutic intervention.

Abbreviation

CCl2

Synonyms

C-C motif chemokine 2; Ccl2; Immediate-early serum-responsive protein JE; MCP-1; Mcp1; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; Scya2; Small-inducible cytokine A2

UniProt

P14844

Accession Number

P14844

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Asn148

Field of Research

Cardiovascular; Cancer; immunology; metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.6 kDa

Observed Molecular Weight

40 kDa

Species

Rat