Recombinant Rat IL-5 protein (His tag)

Specifications

Catalog number

PDMR100026-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 5 (IL-5) is a member of the interleukin family. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.

Abbreviation

IL-5

Synonyms

Interleukin; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; E-CSF; EDF; Eosinophil differentiation factor; ESGF; Interleukin-5; T-cell replacing factor; TRF; Il-5; Il5

UniProt

Q08125

Accession Number

Q08125

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Val132

Field of Research

Cancer; Cardiovascular; Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.41 kDa

Observed Molecular Weight

25 kDa

Species

Rat