Recombinant Rat PCSK9 protein (His tag)

Specifications

Catalog number

PDMR100033-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

Abbreviation

PCSK9

Synonyms

EC 3.4.21; NARC-1; Neural apoptosis-regulated convertase 1; PC9; Proprotein convertase 9; Proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9

UniProt

P59996

Accession Number

P59996

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gln 691

Field of Research

Cardiovascular; Cell Biology; Metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

75.9 kDa

Observed Molecular Weight

60 kDa

Species

Rat