Recombinant Human IL-8/CXCL8 Protein (aa 23-99, Fc Tag)

Specifications

Catalog number

PKSH030279

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental chang. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer.

Abbreviation

IL-8/CXCL8

Synonyms

CXCL; Interleukin; C-X-C motif chemokine; Chemokine (C-X-C motif) ligand; IL8/CXCL; GCP; NAP; CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1;3-10C; AMCF-I; IL8/CXCL8; K60; Interleukin-8; IL-8; Emoctakin; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Granulocyte chemotactic protein 1 (GCP-1) ; Monocyte-derived neutrophil chemotactic factor (MDNCF) ; (Ala-IL-8) 77; (Ser-IL-8) 72;9E3; Beta thromboglobulin like protein; CEF-4; chemokine; CXC motif; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; Granulocyte chemotactic protein 1; IL-8 (1-77) ; IL-8 (9-77) ; IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; Inteleukin 8; ligand 8; Lymphocyte-derived neutrophil-activating factor; MDNCF-b; MDNCF-c; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; Neutrophil activating peptide 1; Neutrophil activating protein 1; Neutrophil-activating factor; Neutrophil-activating protein 1; Protein 3 10C; Protein 3-10C; SCYB8; Small inducible cytokine subfamily B member 8; T cell chemotactic factor; T-cell chemotactic factor; TSG1

UniProt

P10145

Accession Number

NP_000575.1

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Ala 23-Ser 99

Field of Research

Cardiovascular; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.6 kDa

Observed Molecular Weight

40 kDa

Species

Human