Recombinant Human CD16a/FCGR3A Protein (176 Val, His Tag)

Specifications

Catalog number

PKSH030286-01

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

Synonyms

CD16; CD16A; CD16a; CD16a Antigen; FCG3; FCGR3; FCGR3A; Fc-Gamma RIII; Fc-Gamma RIII-Alpha; Fc-gamma RIIIa; FcR-10; FcRIII; FcRIIIa; IGFR3; IgG Fc Receptor III-2; Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A

UniProt

P08637

Accession Number

AAH17865.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Gln 208

Applications

BLI; ELISA

Field of Research

Immunology, Stem Cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

1. Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 80nM. 2. Immobilized human CD16a-His (176 Val) at 10ug/ml (100ul/well) can bind human IgG1 with a linear range of 0.00128-0.8 μg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.3 kDa

Observed Molecular Weight

48 kDa

Species

Human