Recombinant Human CD32a/FCGR2A Protein (167 His, His&AVI Tag), Biotinylated

Specifications

Catalog number

PKSH030290-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a, FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production.

Synonyms

CD32; CD32A; CDw32; FCG2; FCGR2 ; FCGR2A; FCGR2A1; Fc gamma RIIA; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FcRII-a; IGFR2; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt

P12318

Accession Number

P12318-1

Expression System

HEK293 Cells

Conjugation

Biotinylated

Tag

C-His-Avi

Sequence

Met 1-Met 210

Applications

BLI; ELISA

Field of Research

Immunology, Stem Cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM. 3. Labeling ratio of biotin to protein: 0.1-0.6.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.6 kDa

Observed Molecular Weight

33 kDa

Species

Human