Recombinant Human Interleukin-18/IL-18 Protein (GST Tag)

Specifications

Catalog number

PKSH030306

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-18 (IL-18; also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. IL-18 is an IL-1− like cytokine that requires cleavage with caspase-1 to become active; was found to increase IgE production in a CD4+ T cells-; IL-4− and STAT6− dependent fashion. IL-18 and T cell receptor− mediated stimulation could induce naï ve CD4+ T cells to develop into IL-4− producing cells in vitro. Thus; caspase-1 and IL-18 may be critical in regulation of IgE production in vivo; providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was; in turn; upregulated by TNF-α and IL-1β ; suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides; synergistic combinations of IL-18; IL-12; and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

Abbreviation

Interleukin-18/IL-18

Synonyms

MGC; IL1F; IL-1F; IL18; IGIF; IL-18; IL-1g; IL1F4; GIF; Iboctadekin; IFN-gamma-inducing factor; IL-1 gamma; IL-1F4; Interferon gamma-inducing factor; Interferon-gamma-inducing factor; Interleukin 18; Interleukin-1 gamma; Interleukin-18; MGC12320; IFN gamma inducing factor; IL 1 gamma; IL 18; IL 1g; IL1 gamma; IL18 protein; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interleukin 1 gamma; Interleukin 18 (interferon-gamma-inducing factor) ; Interleukin18

UniProt

Q14116

Accession Number

Q14116

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-Asp 193

Field of Research

Cancer; immunology; metabolism

Endotoxin

Please contact us for more information.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

48.6 kDa

Species

Human