Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein

Specifications

Catalog number

PKSH030312

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

AMPK; AMPKa1

UniProt

P54619; Q9Y478; P54646

Accession Number

P54619&Q9Y478&P54646

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Pro 331&Met 1-Ile 272&Met 1-Arg 552

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

38&30&90 kDa

Observed Molecular Weight

35&37&95 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% glycerol, 1mM GSH, pH 7.4

Species

Human