Recombinant Human AMPK (G1/B1/A1) Heterotrimer Protein

Specifications

Catalog number

PKSH030314

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

AMPK; AMPKa1

UniProt

P54619-1; Q9Y478-1; Q13131-1

Accession Number

P54619-1&Q9Y478-1&Q13131-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Pro 331&Met 1-Ile 270&Met 1-Gln 559

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 94 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

38&30&92 kDa

Observed Molecular Weight

38&40&95 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% glycerol, pH 7.4

Citation 01

https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1878-0261.13211

Species

Human