Recombinant Human SPHK1/Sphingosine Kinase 1 Protein

Specifications

Catalog number

PKSH030315

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SPHK1; also known as sphingosine Kinase 1; catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P) . S1P exhibits a broad spectrum of biological activities including cell proliferation; survival; migration; cytoskeletal organization; and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory; antiapoptotic; and immune processes.

Synonyms

SPHK; SPHK1; SPK

UniProt

Q9NYA1

Accession Number

Q9NYA1-1

Expression System

Baculovirus-Insect Cells

Tag

None

Sequence

Met 1-Leu 384

Applications

Enzyme

Field of Research

Signal Transduction, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

42.7 kDa

Observed Molecular Weight

46 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0

Species

Human