Recombinant Human PFK1/PFKM Protein (His & GST Tag)

Specifications

Catalog number

PKSH030320

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.

Synonyms

6-phosphofructokinase; ATP-PFK; GSD7; PFK-1; PFK1; PFKA; PFKM; PFKX; PPP1R122; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphohexokinase; muscle type

UniProt

P08237

Accession Number

P08237-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Thr 2-Val 780

Field of Research

Signal Transduction, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

112.9 kDa

Observed Molecular Weight

113 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol

Species

Human