Recombinant Human ROR1 Protein (aa 453-783, His & GST Tag)

Specifications

Catalog number

PKSH030321

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptor tyrosine kinase-like orphan receptor 1 (ROR1), also known as neurotrophic tyrosine kinase, it is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. ROR1 expressed strongly in human heart, lung and kidney, but weakly in the CNS. At developmental stage, it expressed at high levels during early embryonic development. ROR1 has been shown to have very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling.

Synonyms

NTRKR1; dJ537F10.1

UniProt

Q01973

Accession Number

AAA60275.1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 453-Asn783

Applications

Enzyme

Field of Research

Signal Transduction, Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 0.3 nmol/min/mg using MBP as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

65.3 kDa

Observed Molecular Weight

63 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 2mM GSH, 3mM DTT, 10% glycerol, pH 7.4

Species

Human