Recombinant Human EphA2 Protein (aa 585-976, His & GST Tag)

Specifications

Catalog number

PKSH030323

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Eph receptor A2 is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors' corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2 (EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2 (EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character.

Synonyms

ARCC2; CTPA; CTPP1; CTRCT6; ECK; EPHA2; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK

UniProt

P29317

Accession Number

P29317

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Leu 585-Ile 976

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 50 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

72.1 kDa

Observed Molecular Weight

62 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 3mM DTT, pH 8.5, 10% glycerol

Species

Human