Recombinant Human CKMT1A Protein (His Tag)

Specifications

Catalog number

PKSH030325

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CKMT1A belongs to the ATP:guanido phosphotransferase family. It contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. CKMT1A gene is one of two genes which encode the ubiquitous mitochondrial creatine kinase (CKMT1) . CKMT1 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. CKMT1 occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric CKMT1.

Synonyms

CKMT; CKMT1; CKMT1A

UniProt

P12532

Accession Number

P12532-1

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Ala 40-His 417

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

45.3 kDa

Observed Molecular Weight

43 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol

Species

Human