Recombinant Human ADK Protein (His & GST Tag)

Specifications

Catalog number

PKSH030331

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adenosine kinase (ADK) belongs to the family of transferases. Adenosine kinase (ADK) is the key enzyme in adenosine metabolism and catalyzes ATP and adenosine into two products: ADP and AMP. Two isoforms of the enzyme adenosine kinase (ADK), which differ at their N-terminal ends, are found in mammalian cells. It has been shown that the two ADK isoforms differ only in their first exons and the promoter regions; hence they arise via differential splicing of their first exons with the other exons common to both isoforms. In adult brain, ADK is primarily present in astrocytes. Several lines of experimental evidence support a critical role of ADK in different types of brain injury associated with astrogliosis, which is also a prominent morphologic feature of temporal lobe epilepsy (TLE) . It has been suggested that dysregulation of ADK in astrocytes is a common pathologic hallmark of TLE. Moreover, in vitro data suggest the existence of an additional layer of modulatory crosstalk between the astrocyte-based adenosine cycle and inflammation. ADK also contributes to CK homeostasis in vivo.

Synonyms

AK

UniProt

P55263

Accession Number

AAH03568.1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-His 345

Field of Research

Signal Transduction, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

68.0 kDa

Observed Molecular Weight

60 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 0.3mM DTT

Species

Human