Recombinant Human CSNK1G2 Protein (His Tag)

Specifications

Catalog number

PKSH030337

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Casein kinase I gamma 2 isoform (CSNK1G2) ; a member of the large casein kinase I (CKI) subfamily; protein kinase superfamily. It may affect the development of brain; and associate with vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. The CKI family includes several other isoforms (alpha; beta; gamma; and delta) . Dishevelled (Dsh) ; another positive component of the Wnt pathway; becomes phosphorylated in response to Wnt signals. All the CKI isoforms; with the exception of gamma; increase the phosphorylation of Dsh in vivo. Casein kinase 1 gamma (CK1gamma; or CSNK1G) is associated with the cell membrane and binds to LRP. CK1gamma was found to be needed for Wnt signaling through Wnt receptor LRP. CSNK1G2 inhibits Smad3-mediated TGF-beta responses including induction of target genes and cell growth arrest; and this inhibition is dependent on CSNK1G2 kinase activity. The overexpression of CSNK1G2 in human cancers; may act as an oncoprotein during tumorigenesis. In addition; as an MTA1s-binding protein; CSNK1G2 could further potentiate the estrogen receptor (ER) corepressive function of MTA1s.

Synonyms

CK1G2; CSNK1G2; Casein kinase I isoform gamma-2

UniProt

P78368

Accession Number

P78368-1

Expression System

Baculovirus-Insect Cells

Tag

N-His

Sequence

Met 18-Lys 415

Applications

Enzyme

Field of Research

Signal Transduction, Stem Cells, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 13 nmol/min/mg using casein as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

47.8 kDa

Observed Molecular Weight

48 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, 1mM DTT, pH 8.0

Species

Human