Recombinant Human TLK1/PKU-beta Protein (His & GST Tag)

Specifications

Catalog number

PKSH030339

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.

Synonyms

KIAA0137; PKU-beta

UniProt

Q9UKI8

Accession Number

Q9UKI8-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Tyr 766

Applications

Enzyme

Field of Research

Epigenetics and Nuclear Signaling, Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 88 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 1. 6 nmol/min/mg using Histone H3 protein as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

114 kDa

Observed Molecular Weight

125 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol

Species

Human