Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)

Specifications

Catalog number

PKSH030346

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The encoded protein contains two functional catalytic domains; a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR) . The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins; regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.

Synonyms

IRE1; IRE1P; IRE1a; hIRE1p

UniProt

O75460

Accession Number

O75460-1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Pro 465-Leu 977

Applications

Enzyme

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 5 nmol/min/mg using MBP as substrate

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

86.0 kDa

Observed Molecular Weight

90 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4

Species

Human