Recombinant Human MAP4K2/GC Kinase Protein (His & GST Tag)

Specifications

Catalog number

PKSH030347

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mitogen-activated protein kinase kinase kinase kinase 2, also known as B lymphocyte serine/threonine-protein kinase, Germinal center kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, Rab8-interacting protein and MAP4K2, is a cytoplasm and peripheral membrane protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K2 contains oneCNH domain and oneprotein kinase domain. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. MAP4K2 can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. It is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1 / MEKK1. MAP4K2 enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. It may also play a role in the regulation of vesicle targeting or fusion.

Synonyms

BL44; GCK; MAP4K2; RAB8IP

UniProt

Q12851

Accession Number

AAH47865.1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Tyr 812

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 87 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

119 kDa

Observed Molecular Weight

116 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0

Species

Human