Recombinant Human c-Yes/YES1 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030348

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proto-oncogene tyrosine-protein kinase Yes, also known as Proto-oncogene c-Yes, p61-Yes and YES1, is a cytoplasm protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and SRC subfamily. YES1 / c-Yes contains oneprotein kinase domain, oneSH2 domain and oneSH3 domain. It is thought that the subcellular distribution of Src-family tyrosine kinases, including c-Yes binding to the cellular membrane, is membranous and/or cytoplasmic. YES1 / c-Yes protein tyrosine kinase is known to be related to malignant transformation. YES1 / c-Yes and c-Src are the two most closely related members of the Src family of nonreceptor tyrosine kinases. Although there is much evidence to support redundancy in signaling between these two kinases. YES1 / c-Yes promotes formation of the tight junction by phosphorylating occludin, while c-Src signaling downregulates occludin formation in a Raf-1 dependent manner. YES1 / c-Yes has tyrosine kinase activity. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP / CD46.

Synonyms

HsT441; P61-YES; Yes; c-yes

UniProt

P07947

Accession Number

NP_005424.1

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Gly 2-Leu 543

Applications

Enzyme

Field of Research

Signal Transduction; Neuroscience; Cancer; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 35 nmol/min/mg using Poly (Glu, Tyr) 4:1 as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

88.5 kDa

Observed Molecular Weight

75 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, 0.5mM TCEP, pH 8.0

Species

Human