Recombinant Human GRK6/GPRK6 Protein (His & GST Tag)

Specifications

Catalog number

PKSH030350

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

G protein-coupled receptor kinase 6, also known as G protein-coupled receptor kinase GRK6, GRK6 and GPRK6, is a lipid-anchor protein which belongs to theprotein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRK6 / GPRK6 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. This protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described. GRK6 / GPRK6 is widely expressed. GRK6 / GPRK6 silencing causes suppression of signal transducer and activator of transcription 3 (STAT3) phosphorylation associated with reduction in MCL1 levels and phosphorylation, illustrating a potent mechanism for the cytotoxicity of GRK6 inhibition in multiple myeloma (MM) tumor cells. GRK6 also appears to be involved in responses to morphine. Inhibition of GRK6 represents a uniquely targeted novel therapeutic strategy in human multiple myeloma.

Synonyms

GPRK6

UniProt

P43250

Accession Number

P43250-2

Expression System

Baculovirus-Insect Cells

Tag

N-His-GST

Sequence

Met 1-Arg 589

Applications

Enzyme

Field of Research

Signal Transduction, Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 93 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 7 nmol/min/mg using casein as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

95.1 kDa

Observed Molecular Weight

85 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 2mM GSH, 0.5mM PMSF, pH 7.4

Species

Human