Recombinant Human STK10/LOK Protein (His Tag)

Specifications

Catalog number

PKSH030352

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine / threonine-protein kinase 10, also known as Lymphocyte-oriented kinase, STK10 and LOK, which belongs to theprotein kinase superfamily, STE Ser / Thr protein kinase family and STE20 subfamily. Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells. The Ste20 family of serine/threonine kinases plays an important role in numerous cellular functions such as growth, apoptosis, and morphogenesis. STK10 is similar to several known polo-like kinase kinases. It can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. STK10 can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. Stk10 can associate with Plk1 in cells and furthermore can phosphorylate Plk1. It can also act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.

Synonyms

LOK; PRO2729

UniProt

O94804

Accession Number

NP_005981.3

Expression System

E.coli

Tag

N-His

Sequence

Arg 18-Glu 317

Applications

Enzyme

Field of Research

Signal Transduction

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

The specific activity was determined to be 1353 nmol/min/mg using synthetic AXLtide peptide (KKSRGDYMTMQIG) as substrate.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

36.0 kDa

Observed Molecular Weight

40 kDa

Formulation

Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0

Species

Human