Recombinant Human IDO1/IDO Protein (Gly&Ala2-Gly403)

Specifications

Catalog number

PKSH030356

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.

Synonyms

3-dioxygenase;3-dioxygenase 1; IDO; IDO-1; IDO1; INDO; Indole 2; Indoleamine 2

UniProt

P14902

Accession Number

P14902-1

Expression System

E. coli

Tag

N-His

Sequence

Ala 2-Gly 403

Applications

Enzyme

Field of Research

Signal Transduction, Cardiovascular, Metabolism

Endotoxin

Please contact us for more information.

Purity

> 88 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to oxidize L-tryptophan to N-formylk-ynurenine. The specific activity is > 400 pmoles/min/μg.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

46.0 kDa

Observed Molecular Weight

44 kDa

Formulation

Supplied as sterile solution of 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0

Species

Human