Recombinant Human NME1/NDKA Protein (His Tag)

Specifications

Catalog number

PKSH030357

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.

Synonyms

AWD; GAAD; Granzyme A-Activated DNase; Metastasis Inhibition Factor nm23; NB; NBS; NDK A; NDKA; NDP Kinase A; NDPK-A; NDPKA; NM23; NM23-H1; NME1; Nucleoside Diphosphate Kinase A; Tumor Metastatic Process-Associated Protein; nm23-H1

UniProt

P15531

Accession Number

NP_000260.1

Expression System

E.coli

Tag

N-His

Sequence

Ala 2-Glu 152

Field of Research

Cancer; epigenetics and nuclear signal

Endotoxin

Please contact us for more information.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

18.0 kDa

Observed Molecular Weight

21 kDa

Formulation

Supplied as sterile solution of PBS, pH 7.4

Species

Human